![]() Owing to the frequently high relatedness between the ancestral progenitors, multiple alleles are often present in more than one copy. Analyses of polymorphic markers such as microsatellites, however, are hindered in allopolyploid species because of the presence of more than two alleles at each locus and the resulting complexity of the inheritance pattern (as reviewed in De Silva et al., 2005 Ronfort et al., 1998).Īllopolyploids have differentiated pairs of chromosomes (homeologous chromosomes) which form bivalents at meiosis, as in their diploid progenitors. Over previous decades, microsatellites have emerged as markers of choice in such analyses because of their high level of polymorphism and their codominant inheritance ( Powell et al., 1996). serotina is economically important in North America for its timber quality ( Marquis, 1990) and ecologically important in Europe owing to its invasive status, questions regarding fine-scale spatial genetic structure, mating system, or gene flow among populations in both its native and introduced ranges are of interest to foresters and ecologists. Prunus serotina has been classified in subgenus Padus (Miller) Focke ( Rehder, 1940), and molecular phylogenetic studies of Prunus place the species in a clade composed of members of that subgenus as well as subgenus Laurocerasus (Duh.) Rehder ( Bortiri et al., 2006). Prunus serotina is a wild, fast-growing tree native to North America ( Marquis, 1990) which has been widely planted in European forests and has spread from plantations to invade several types of woodlands and open habitats on poor soils ( Muys et al., 1992). Keywords: polyploids SSR cross-amplification Rosaceae black cherry ![]() The potential use of these genome-specific markers in population genetic studies is discussed. We found both a high number of alleles per locus (three to 12) and a high mean expected heterozygosity (0.71), which were nonsignificantly different from the number of alleles and estimates of expected heterozygosity calculated for three non-genome-specific markers in the same population. No linkage disequilibrium was detected for these loci, but evidence was found for the presence of a null allele at one locus. Among the 67 microsatellite markers tested, 26 produced successful amplification and five were genome-specific. serotina, and evidence that conserved markers were genome-specific was found by demonstrating their typical Mendelian diploid inheritance in embryos resulting from controlled crosses. species were tested for cross-amplification in P. Sixty-seven microsatellite primers described in cultivated Prunus L. by finding genome-specific primers (i.e., primers that are specific to one of the two genomes that initially formed the species). The objective of this study was to solve these problems in the allotetraploid Prunus serotina Ehrh. The utility of microsatellite markers to characterize the genetic diversity of a polyploid species with disomic inheritance is often hampered by the impossibility of determining allele frequencies and the complexity of inheritance patterns.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |